Preparation of a sulfurtransferase substrate, sodium 3-mercaptopyruvate, from 3-bromopyruvic acid and sodium hydrosulfide.
نویسندگان
چکیده
منابع مشابه
Expression of 3-Mercaptopyruvate Sulfurtransferase in the Mouse.
3-Mercaptopyruvate sulfurtransferase (MST) is one of the principal enzymes for the production of hydrogen sulfide and polysulfides in mammalians, and emerging evidence supports the physiological significance of MST. As a fundamental study of the physiology and pathobiology of MST, it is necessary to establish the tissue distribution of MST in mice. In the present study, the expression of MST in...
متن کاملFunctional Analysis of 3-Mercaptopyruvate Sulfurtransferase Using Knockout Mice
3-mercaptopyruvate sulfurtransferase (MST) is a universal enzyme in prokaryotes and eukaryotes. In 1998, we reported the distribution of MST in rat [1]. MST was expressed in numerous somatic cells and was found to be present in both cytosol and mitochondria [1] (Figure 1a). In the kidney, MST levels were higher in the cortex and proximal convoluted tubules as compared to that in the medulla and...
متن کاملSseA, a 3-mercaptopyruvate sulfurtransferase from Escherichia coli: crystallization and preliminary crystallographic data.
SseA, the translation product of the Escherichia coli sseA gene, is a 31 kDa protein endowed with 3-mercaptopyruvate:cyanide sulfurtransferase activity in vitro. As such, SseA is the prototype of a sulfurtransferase subfamily distinguished from the better known rhodanese sulfurtransferases, which display thiosulfate:cyanide sulfurtransferase activity. The physiological role of the two homologou...
متن کاملThioredoxin and dihydrolipoic acid are required for 3-mercaptopyruvate sulfurtransferase to produce hydrogen sulfide.
H2S (hydrogen sulfide) has recently been recognized as a signalling molecule as well as a cytoprotectant. We recently demonstrated that 3MST (3-mercaptopyruvate sulfurtransferase) produces H2S from 3MP (3-mercaptopyruvate). Although a reducing substance is required for an intermediate persulfide at the active site of 3MST to release H2S, the substance has not been identified. In the present stu...
متن کاملarylaminotetrazoles : via [2+3] cycloaddition of nitriles and sodium azide via solid acid catalysis
The [3+2] cycloaddition between various nitriles and sodium azide proceeds smoothly in the presence of zeolite and sulfated Zircona as effective catalyst and in the water and DMF/MeOH, to give the corresponding arylaminotetrazoles in good to high yields. The reaction most probably precedes through the in situ formation of a catalyst azide species, followed by a successive [3+2] cycloaddition wi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1989
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.37.2843